From IADR:
0755 Metabolic changes of cells caused by oral galvanism
W. NIEDERMEIER1, A. GROB1, N. WIRTZ1, H. SAUER2, M. WARTENBERG3, and J. HESCHELER1, 1University of Cologne, Germany, 2University of Giessen, Germany, 3University of Potsdam, Germany
Objective: Electrostatic potentials up to 800 mV can be observed between different dental alloys used for restorative or prosthetic treatment. Associated symptoms are impaired taste, glossodynia and acute or chronic alterations of the oral mucosa. Methods: To investigate physiologic reactions of oral mucosa cells caused by electrostatic corrosion potentials, cell lines (UMSCC-14-C, Heidelberg cell bank) were incubated in a newly developed non polarizing pseudo-realistic electric field ?? device and exposed to voltages of 0-400 mV for a period of 24 hours. Following this, the cells were treated with different antibodies and stained to prove metabolic changes: Keratin-14, Actin, Microtubuli, Ki-67, Dichlorofluorescein, NADPH-oxidase, Catalase, Superoxide-dismutase, Caspase-3, Poly-ADP-Ribose Polymerase. Findings were assessed utilizing laser-scanning microscopy, and data was evaluated statistically using the t-test. Results: Voltages of 200 mV led to cell apoptosis with significant destruction of the cytoskeleton and reduced cell proliferation. As trigger for the connected metabolic changes, a significant (p=0.0064) increase of the reactive oxygen intermediates (ROI) caused by activation of NADPH-oxidase could be observed. Also Superoxide-dismutase and Catalase were increased, but could not improve cellular defence mechanisms sufficiently. Conclusion: Electrostatic potentials of 200 mV stimulate reactive oxygen intermediates that inhibit cell proliferation and result in cell apoptosis. Since mechanisms of cellular defence are activated to an inadequate degree, cells cannot be protected against irreversible damage.
Seq #98 – Oral Biology and Pathology
2:00 PM-3:15 PM, Thursday, March 22, 2007 Ernest N. Morial Convention Center Exhibit Hall I2-J
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